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Objectives

• Upon Completion of the Course, Participants will be able to:
  • The aim is to provide first hand information to the participants on optimal use of gas chromatograph (GC).
  • A special emphasis has been given on the demonstration of applica­tions, maintenance and trouble­shooting of GC.
  • Imparting participants’ fundamen­tal knowledge of Gas Chromatog­raphy as a technique.
  • To understand GC maintenance methods as a routine checks.
  • Knowledge of accessories and con­sumables required for its opera­tion.
  • To familiarize participants to the techniques for achieving gas chro­matography analysis, qualitative and quantitative methods, cause and effect diagrams and standard curve.

The Delegates

• All who works in any laboratory, i.e. clinical, industry, gas, petroleum, refinery, phar­macy, etc.
  • Laboratory manager and supervisor
  • Laboratory engineers and chemists
  • Laboratory analysts and technicians
  • Instrument engineers/technicians

The Contents

• Theory Parameters:
  • Plate height(H),
  • Retention Time,
  • Capacity Fac­tor,
  • Selectivity Factor,
  • Column Efficiency,
  • Column Resolution.
• Sampling And Sample Handling Iso 17025:
  • Requirements for Gases,
  • Liquid and Solid,
  • Sample Sampling Methods,
  • Sample Registra­tion and Storage,
  • Sample Disposal,
  • Max Holding Time of Sample,
  • Sample Contamination.
• Sample Preparation And Analyte Manipulation Technique:
  • Solid-phase extraction,
  • Static head space technique,
  • Soxhlet extraction,
  • Microwave-assisted extraction,
  • Super critical fluid extraction
• Derivatization Method:
  • Silyl,
  • Alkyl,
  • Acylation and Esterfication Methods
• Types Of GC:
  • Normal GC,
  • High Speed GC and Portable GC
• Gc Separation And Retention Process:
  • Dispersive Interac­tions,
  • Dipole-Dipole Interactions,
  • Dipole-Induced Dipole Interactions
• Carrier Gas:
• Van Deemter equation,
  • Optimal Practical Gas Velocity(OPGV),
  • Average Linear Velocity,
  • Flow Rate,
  • Carrier Gas Selection,
  • Pressure Regulator,
  • Gas Purification
• Sample Injection System:
  • Sample Introduction Technique,
  • Manual Injection,
  • Solvent Flush Injection,
  • Septa,
  • Inlet Liner,
  • Split/Split less Inlets System,
  • Spit Ratio,
  • Purge Delay Time,
  • Cool On-Column Inlets,
  • Programmed Temperature Vaporization,
  • Headspace Technique,
  • Injection Problems,
  • Hot Vaporizing Injector
• Column Configurations:
  • Stationary Phase of Packed and Capillary Column,
  • Packed Columns,
  • Temperature programming,
  • Gas-Liquid and Gas- Solid Columns,
  • Polarity and Selectivity,
  • Column Coating
• Detectors:
  • Ideal Characteristics,
  • Column Position,
  • Gases,
  • Flame Ionization Detector,
  • Thermal Conductivity Detector,
  • Flame Photometric Detector,
  • Nitrogen Phosphorous Detector,
  • Photo Ionization Detector,
  • Electron Capture Detector,
  • Mass Spectrometry(Fragmentation and identify technique with software training),
  • Chemiluminescent Detector,
  • Mass Analyzer – Time of Flight,
  • Ion Trap and Quadrupole Technique.
• Maintenance And Installation Methods:
  • Clean and Condition Septa,
  • Cleaning Injector Liners,
  • Silylating Liners,
  • Column Conditioning,
  • Installation Fused Silica Capillary Columns,
  • Column Placement in the oven,
  • Column Installation,
  • Leak Detection,
  • Bleed Test,
  • FidJet Cleaning Procedure,
  • TCD Clean Detector Cell,
  • Cleaning Of ECD,
  • FPD Mainte­nance,
  • Cleaning MS and Change The Filament
• Instrument Problems And Troubleshooting:
  • Approaches to Solve GC Problems,
  • Troubleshooting Tests,
  • Band Broadening,
  • Broadening Initial Peak Band widths,
  • Retention Gap Sampling,
  • Sampling By Solute Fo­cusing,
  • Retention Gaps Tube,
  • Baseline Deviation,
  • Noisy Baseline,
  • Spikes in Baseline,
  • Peak Shape Problems,
  • Flat Top Peaks,
  • Split Peaks,
  • Negative Peaks,
  • Retention Changes,
  • Ghost Peak,
  • Causes and Prevention of Column Damage,
  • Column Contamination,
  • Needle Discrimination,
  • Change in Detec­tors Sensitivity,
  • Difficulty in Lighting Fid Flame,
  • Loss of Detectors Linear Range,
  • Leaks in MS,
  • Excessive Noise or High Background in MS
• Practical Demonstration:
  • Introduction and familiarization with GC,
  • Preparation of sample test,
  • Preparation of standards,
  • Setting the methods program,
  • Inject the sample and standards,
  • Print the chromatograms,
  • Check all GC components and how to do the preventive maintenance
• Quality Control And Validation Method:
  • Specificity,
  • Linear­ity,
  • Selectivity,
  • Accuracy,
  • Precision,
  • Bias,
  • Linearity,
  • Range,
  • Limit of De­tection,
  • Limit Of Quantification,
  • Robustness,
  • Ruggedness,
  • Stability
• Quantitative Methods:
  • Interpolated Graph Calibration by Using External and Internal Standards,
  • Standard Addition Method,
  • Calibration Methods,
  • Correlation Coefficient,
  • Outliers Test of Repeated Measure­ments,
  • Outliers In Calibration Curve,
  • Errors In Quantitative Analysis,
  • Distribution of Errors,
  • Confidence Limit,
  • Limit of Detection,
  • Repeatabil­ity,
  • Reproducibility,
  • Optimization Methods
• Course summary .

 Notes:

• Great Discount for companies and governmental Organizations .
• All programs are held in five star hotels .
• All lecturers have sufficient knowledge and experience to implement the programs at an optimal level .
• Large package of services is offered to the participants .

The Discount

10% in case of Three P. (or more)